Over Expression:Article Title: SATB2 accelerates post-traumatic stress disorder (PTSD) via increased FOXM1/STAT3/P2X7R signaling-induced inflammation in the hippocampus.
Article Snippet: .. Antibodies against SATB2, FOXM1, p-STAT3, P2X7R, IL-6, TNF-α, IL-1β, and GAPDH (glyceraldehyde-3-phosphate dehydrogenase), as well as secondary antibodies (goat anti-mouse IgG, goat anti-rabbit IgG), were purchased from Abcam.OE (overexpression) and P2X7R-KD (knockdown) lentiviruses were obtained from Shanghai Jiman Biotechnology.FOXM1 inhibitor Thiostrepton, STAT3 agonist Colivelin TFA (TFA), and P2X7R antagonist AZ10606120 dihydrochloride (AD) were sourced from MCE (USA).An R500 small animal anesthesia machine was purchased from RWD Life Science (China).The primary reagents used in this study include: anti-SATB2, FOXM1, p-STAT3, P2X7R, IL-6, TNF-α, IL-1β, and GAPDH antibodies, along with corresponding horseradish peroxidase-labeled secondary antibodies (goat anti-mouse IgG, goat anti-rabbit IgG), all purchased from MCE Inc.in the United tates; SATB2 overexpression (SATB2-OE) and P2X7R knockdown (P2X7R-KD) lentiviruses were constructed by Shanghai Jiman Biotechnology Co., Ltd., both carrying a green fluorescent protein (GFP) tag; FOXM1 inhibitor Thiostrepton, STAT3 agonist Colivelin TFA, and P2X7R antagonist AZ10606120 dihydrochloride were purchased from MCE, Inc.(USA); The R500 small animal anesthesia machine was purchased from Ruiwo Life Sciences Co., Ltd. ..
Knockdown:Article Title: SATB2 accelerates post-traumatic stress disorder (PTSD) via increased FOXM1/STAT3/P2X7R signaling-induced inflammation in the hippocampus.
Article Snippet: .. Antibodies against SATB2, FOXM1, p-STAT3, P2X7R, IL-6, TNF-α, IL-1β, and GAPDH (glyceraldehyde-3-phosphate dehydrogenase), as well as secondary antibodies (goat anti-mouse IgG, goat anti-rabbit IgG), were purchased from Abcam.OE (overexpression) and P2X7R-KD (knockdown) lentiviruses were obtained from Shanghai Jiman Biotechnology.FOXM1 inhibitor Thiostrepton, STAT3 agonist Colivelin TFA (TFA), and P2X7R antagonist AZ10606120 dihydrochloride (AD) were sourced from MCE (USA).An R500 small animal anesthesia machine was purchased from RWD Life Science (China).The primary reagents used in this study include: anti-SATB2, FOXM1, p-STAT3, P2X7R, IL-6, TNF-α, IL-1β, and GAPDH antibodies, along with corresponding horseradish peroxidase-labeled secondary antibodies (goat anti-mouse IgG, goat anti-rabbit IgG), all purchased from MCE Inc.in the United tates; SATB2 overexpression (SATB2-OE) and P2X7R knockdown (P2X7R-KD) lentiviruses were constructed by Shanghai Jiman Biotechnology Co., Ltd., both carrying a green fluorescent protein (GFP) tag; FOXM1 inhibitor Thiostrepton, STAT3 agonist Colivelin TFA, and P2X7R antagonist AZ10606120 dihydrochloride were purchased from MCE, Inc.(USA); The R500 small animal anesthesia machine was purchased from Ruiwo Life Sciences Co., Ltd. ..
Construct:Article Title: SATB2 accelerates post-traumatic stress disorder (PTSD) via increased FOXM1/STAT3/P2X7R signaling-induced inflammation in the hippocampus.
Article Snippet: .. Antibodies against SATB2, FOXM1, p-STAT3, P2X7R, IL-6, TNF-α, IL-1β, and GAPDH (glyceraldehyde-3-phosphate dehydrogenase), as well as secondary antibodies (goat anti-mouse IgG, goat anti-rabbit IgG), were purchased from Abcam.OE (overexpression) and P2X7R-KD (knockdown) lentiviruses were obtained from Shanghai Jiman Biotechnology.FOXM1 inhibitor Thiostrepton, STAT3 agonist Colivelin TFA (TFA), and P2X7R antagonist AZ10606120 dihydrochloride (AD) were sourced from MCE (USA).An R500 small animal anesthesia machine was purchased from RWD Life Science (China).The primary reagents used in this study include: anti-SATB2, FOXM1, p-STAT3, P2X7R, IL-6, TNF-α, IL-1β, and GAPDH antibodies, along with corresponding horseradish peroxidase-labeled secondary antibodies (goat anti-mouse IgG, goat anti-rabbit IgG), all purchased from MCE Inc.in the United tates; SATB2 overexpression (SATB2-OE) and P2X7R knockdown (P2X7R-KD) lentiviruses were constructed by Shanghai Jiman Biotechnology Co., Ltd., both carrying a green fluorescent protein (GFP) tag; FOXM1 inhibitor Thiostrepton, STAT3 agonist Colivelin TFA, and P2X7R antagonist AZ10606120 dihydrochloride were purchased from MCE, Inc.(USA); The R500 small animal anesthesia machine was purchased from Ruiwo Life Sciences Co., Ltd. ..
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